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SAUER LAB

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Contents

Expression

Materials Needed

Protocol

Day One

Day Two

Purification

Materials Needed

Clp Buffer:

Lysis Buffer (1L)

Wash buffer

Adjust to pH 8

Elution buffer

Adjust to pH 8

Q Loading Buffer 1L

Q Elution Buffer 1L

Purification

Ni-NTA

MonoQ